The Center for Molecular and Genomic Imaging at the UC Davis Department of Biomedical Engineering, contributed X-ray computed tomography scanning on a 0.53g fragment of Chelyabinsk, C3-3-4, using the Center’s MicroXCT-200 specimen CT scanner (Xradia Inc.). A video made from the images obtained by CMGI are here: UC Davis website at http://www.youtube.com/user/YinLabatUCDavis or at http://www.youtube.com/user/CMGIPreclinical/. Full story here: http://bmestage.engineering.ucdavis.edu/blog/cmgi-did-x-ray-ct-scanning-for-first-study-of-chelyabinsk-meteorite/
Pilot Studies Program
CMGI has a limited amount of funding available to support pilot projects! These pilot funds are expected to be used to generate preliminary data for grant applications. Funding can be used for imaging or radiochemistry development projects and can be used to cover imaging time, radionuclide production, radiotracer synthesis, contrast agents and data analysis. Each grant will typically be in the $2,000 – $4,000 range, although applications up to a maximum of $10,000 will be considered, particularly for investigators developing new radiotracers. Further information and applications are available at Pilot Studies.
FREE Introductory Use of MicroXCT-200 Specimen Scanner
Investigators are invited to scan 2 specimens, which may include multiple scans of a single specimen, at no charge until January, 2013. Subsequent specimen scans will be recharged at the approved Specialty Equipment and Labor rates (http://imaging.bmestage.engineering.ucdavis.edu/faqs/recharge-rates/). The Labor rate will only be charged for the setup time, typically 0.5-1 hr. The MicroXCT is a high resolution scanning instrument that can achieve 1-5 mm resolution in samples a few centimeters in size. It may be used for both non-biological and ex-vivo biological specimens.
Announcing 3 New Imaging Instruments
The Center for Molecular and Genomic Imaging (CMGI), one of two facilities comprising the Shared Animal Imaging Resource, is receiving three new state-of-the-art imaging instruments funded with grants totaling more than $1.3M from the NIH Shared Instrument Grant Program (S10). These dedicated small-animal and small-specimen imaging instruments will add significant new capabilities to the Shared Animal Imaging Resource by extending the CMGI’s slate of preclinical imaging modalities, which currently include the in vivo imaging technologies PET, SPECT, MRI, CT, optical and ultrasound imaging.
First, a new IVIS Spectrum (Caliper/PerkinElmer) will replace an older model (IVIS 100; Xenogen) that was primarily used for bioluminescence imaging. The system will be installed in mid 2012. The Spectrum offers improved bioluminescence imaging and adds the capability for advanced fluorescence imaging.
CMGI will acquire a MicroXCT-200 (Xradia) in mid 2012. This system provides researchers with the ability to perform CT imaging of biological specimens with high image contrast down to a resolution of approximately 1-5 micrometers for ultra-high resolution imaging of specimens as large as a few cm.
Lastly, CMGI has just installed an Optical Imaging Cryomicrotome (Barlow Scientific) that is able to acquire high-resolution (10-30 um) fluorescence images of frozen tissue sections or rodent whole bodies. The microtome can cut sections down to 25 micrometers thick from specimens as large as 11cm x 24cm in size. A CCD video camera mounted with motorized excitation and emission filter wheels provides high-resolution fluorescence images for quantitative analysis of fluorescence distribution.
C-11 PET Tracers Now Available
First tracers in routine production bind to dopamine receptors
Raclopride and [11C]SCH23390 are being used in a UC Davis study of the neurological basis of social bonding of the Titi monkey. Above, a transaxial brain image after administration of [11C]raclopride shows selective uptake in the putamen. Image courtesy of Professor Karen L. Bales.
The CMGI Radiochemistry laboratory now produces [11C]SCH23390 and [11C]raclopride, two PET tracers for studying regional in vivo binding of ligands to dopamine receptors. These are the first 11C neuro tracers in routine production at CMGI.
Dopamine plays a pivotal role in the regulation and control of movement, motivation and cognition. PET enables noninvasive direct measurement of components of the dopamine system in the living brain. Selective, high-affinity PET radioligands have been developed to study individual subtypes of dopamine receptors. [11C]SCH23390 and [11C]raclopride are tracers for the most extensively studied dopamine receptor subtypes, D1 and D2/D3, respectively.
To synthesize the PET tracers, 11C is produced on site with the Siemens RDS111 Biomedical cyclotron, and delivered to an automated gas-phase production (GPP) synthesis unit. GPP produces tracers of high specific activity; that is, the mass of tracer per imaging dose is exceptionally low.
Other well established tracers currently available at CMGI include [18F]FDG, [18F]FLT, [18F]FMISO, and [18F]FHBG. Method development is now underway for [11C]PK11195, which has been extensively used in studies of the peripheral benzodiazepine receptor.
As a service to investigators interested in a specific 11C or 18F tracer, CMGI can provide cost estimates for method development and tracer synthesis in support of grant applications. If you are interested in CMGI production of a specific PET tracer, please contact Doug Rowland at email@example.com.